The Scadutree Chalice is located in an area behind the Shadow Keep in Elden Ring's Shadow of the Erdtree DLC. To reach it, players must defeat the boss Commander Gaius and then explore the new area that opens up. The Chalice is found on a precipice overlooking the Scadutree and is surrounded by five Scadutree Fragments, which are useful for leveling up the player's Shadow Blessing. Despite its apparent significance, the exact purpose of the Scadutree Chalice remains a mystery, with various theories proposed by players and fans.
To access the Scadutree Chalice in Elden Ring's Shadow of the Erdtree DLC, players must defeat Commander Gaius, an armored boar-riding boss. After his defeat, players can enter a new area leading to the Scadutree Chalice, where they'll find five Scadutree Fragments scattered around it.
There are no reported instances of players successfully interacting with the Scadutree Chalice beyond collecting the five Scadutree Fragments scattered around it. Speculations about its purpose include a potential location for a secret quest, a site for cleansing the Scadutree, or simply a reward in itself for reaching that area. However, no definitive conclusions have been made.